Networks in the Life Sciences – Genomics, Proteomics and Systems Biology
14th EMBL PhD Symposium

25th–27th October 2012

Speaker: Prof. Dr. Juri Rappsilber

  • Wellcome Trust Centre for Cell Biology, University of Edinburgh
  • Einstein Professor, Technical University of Berlin

Background

Juri Rappsilber Professor at the Wellcome Trust Institute for Cell Biology, Juri Rappsilber combines chemistry, biology & informatics to develop qualitative & quantitative mass spectrometry methods. He uses these methods to study protein–nucleic acid and protein–protein interactions in complex mixtures.

Talk: Proteomics, embracing the chaos

As a PhD student I learnt the three most important indications for the function of an uncharacterised protein: Location, location, location. Or is it? As analysis depth increases so does the number of out-of-context proteins identified at any location in the cell investigated. Of course, analysis by proteomics requires prior isolation of a cellular structure/organelle. Are cells tidy and experiments dirty? Here we analyse chromatin by proteomics and challenge the black/white logic of proteomic lists and GO annotations. Interestingly, this departure shows a more direct root to protein function and may affect our view of the cellular interior.